sanger dna sequencing (Azenta)
86
Structured Review
Azenta
sanger dna sequencing
Sanger Dna Sequencing, supplied by Azenta, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sanger+dna+sequencing/sanger+sequencing/pmc12966454-235-6-9
Average 86 stars, based on 1 article reviews
Sanger Dna Sequencing, supplied by Azenta, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sanger+dna+sequencing/sanger+sequencing/pmc12966454-235-6-9
Average 86 stars, based on 1 article reviews
sanger dna sequencing - by Bioz Stars,
2026-09
86/100 stars
Images
1) Product Images from "Engineering plasmids with synthetic origins of replication"
Article Title: Engineering plasmids with synthetic origins of replication
Journal: Nature Communications
doi: 10.1038/s41467-026-68907-1
Figure Legend Snippet: A Schematic of the MB1 plasmid origin of replication (ORI) mechanism. The RNA primer is transcribed by RNA polymerase (RNAP) and, by default, folds into an RNA structure that is processed by RNase H to create a substrate for DNA polymerase I (Pol I) to initiate DNA replication. The antisense RNA is produced from an overlapping gene, which, if allowed to interact with the RNA primer, induces an alternative fold that does not initiate DNA replication. As the concentration of antisense RNA is proportional to the plasmid copy, this serves as a copy-control negative feedback loop. B Schematic of a refactored pMB1 ORI that separates the RNA primer and antisense RNA gene and introduces inactivating mutations in the P1 promoter sequence. C Shows sequences of mutations to inactivate the P1 promoter encoded inside the RNA primer. (Left graph) Whole-cell fluorescent characterization of the mutant promoters using a fluorescent reporter gene. (Right graph) Relative copy number of plasmids containing the original pMB1 ORI or refactored ORI with an RNA primer containing mutant P1 promoters. Copy number was characterized by encoding a constitutive RFP expression cassette onto the plasmid. Fluorescence characterization was performed (measured in units of fluorescence/optical density (OD) at 600 nm) in E. coli cells. Data show mean values ± SD and individual values of n = 4 biological replicates. Source data for this figure is available in the Source Data file.
Techniques Used: Plasmid Preparation, Produced, Concentration Assay, Control, Sequencing, Mutagenesis, Expressing, Fluorescence
![A SynORI plasmids (pSynORI) can be programmed to convert distinct chemical signals into DNA copy output using transcriptional regulators to control RNA primer transcription. Relationship between relative copy number and chemical input for pSynORI built using (B) Plac (activated by IPTG), C PcymR (activated by cumate), D the ZTP riboswitch (activated by Z), E and the yxjA riboswitch (repressed by 2AP). F Matrix showing the relative copy of a pSynORI that is activated by cumate and repressed by IPTG with inducer titrations. Relative copy number was characterized by encoding a constitutive RFP expression cassette onto the plasmid in E. coli cells (measured in units of fluorescence/optical density [OD] at 600 nm). G Schematic of compatible and inducible pSynORI that converts chemical input signals into a DNA copy, which can be read using sequencing. A pSC101 plasmid with a non-inducible copy number is included as a reference. H The relative copy of pSynORI A and B was calculated using the reference plasmid. Bar plots show the DNA copy ratio of pSynORI A and B. Data in B – E and F shows n = 4 biological replicates, and Data in H show n = 3 biological replicates. All error bands indicate SD. Source data for this figure is available in the Source Data file.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_6454/pmc12966454/pmc12966454__41467_2026_68907_Fig4_HTML.jpg "... be programmed to convert distinct chemical signals into DNA copy output using transcriptional regulators to control RNA ...")
Figure Legend Snippet: A SynORI plasmids (pSynORI) can be programmed to convert distinct chemical signals into DNA copy output using transcriptional regulators to control RNA primer transcription. Relationship between relative copy number and chemical input for pSynORI built using (B) Plac (activated by IPTG), C PcymR (activated by cumate), D the ZTP riboswitch (activated by Z), E and the yxjA riboswitch (repressed by 2AP). F Matrix showing the relative copy of a pSynORI that is activated by cumate and repressed by IPTG with inducer titrations. Relative copy number was characterized by encoding a constitutive RFP expression cassette onto the plasmid in E. coli cells (measured in units of fluorescence/optical density [OD] at 600 nm). G Schematic of compatible and inducible pSynORI that converts chemical input signals into a DNA copy, which can be read using sequencing. A pSC101 plasmid with a non-inducible copy number is included as a reference. H The relative copy of pSynORI A and B was calculated using the reference plasmid. Bar plots show the DNA copy ratio of pSynORI A and B. Data in B – E and F shows n = 4 biological replicates, and Data in H show n = 3 biological replicates. All error bands indicate SD. Source data for this figure is available in the Source Data file.
Techniques Used: Control, Expressing, Plasmid Preparation, Fluorescence, Sequencing
Related Articles
DNA Sequencing:Article Title: Adipocyte Heparan Sulfate Determines Type 2 Diabetes Susceptibility in Mice via FGF1-Mediated Glucose Regulation. Article Snippet: .. All assemblies were verified with Article Title: Engineering plasmids with synthetic origins of replication Article Snippet: .. All assembled plasmids were verified using Article Title: Adipocyte heparan sulfate determines type 2 diabetes susceptibility in mice via FGF1-Mediated glucose regulation Article Snippet: .. All assemblies were verified with Article Title: Supporting Information for Visualizing spatiotemporal dynamics of intercellular mechanotransmission upon wounding Article Snippet: .. All the constructed DNA plasmids were analyzed and confirmed by restriction enzyme digestion and Article Title: Mechanism of Action of Gepotidacin: Well-Balanced Dual-Targeting against Neisseria gonorrhoeae Gyrase and Topoisomerase IV in Cells and In Vitro. Article Snippet: The quinolone resistance-determining regions of GyrA and ParC were amplified by polymerase chain reaction (PCR) using described primers.86 PCR products were amplified using Platinum PCR supermix (Invitrogen, 12532-016) and purified using a PCR cleanup kit (Qiagen, 28106). .. DNA transformation was performed according to the protocol by Dillard.82 Azenta Life Sciences was used to verify successful construction by Article Title: Induced pluripotent stem cells carrying novel APTX mutations presented defective neural differentiation with the accumulation of DNA single-strand breaks Article Snippet: Positively expressed cells were selected with 1 μg/mL puromycin (Gibco, Grand Island, NY) in mTeSR Plus for 48 hours. .. The surviving cells were propagated into clones, collected, and analyzed via Article Title: Mechanism of Action of Gepotidacin: Well-Balanced Dual-Targeting against Neisseria gonorrhoeae Gyrase and Topoisomerase IV in Cells and In Vitro Article Snippet: .. Azenta Life Sciences was used to verify successful construction by Article Title: Profiling Allogeneic HLA-specific B-cell Responses Utilizing a 64-plex Single-HLA Reporter Cell Panel Article Snippet: Double-stranded DNA fragments encoding the following genes were synthesized (Gene Universal Inc. or Twist Bioscience) based on their published amino acid sequences, including EBFP2 [ ], mTurquoise2 [ ], LSSmOrange [ ], hmKeima8.5 [ ], NowGFP [ ], mKelly1 [ ], the neomycin-resistance gene (GenBank V00618.1 , with R177S mutation), the hygromycin-resistance gene (UniProt P00557), human CD74 (GenBank NM_001025159.2), and 73 unique HLA alleles (IPD-IMGT/HLA Database [ ]) listed in . .. All recombinant plasmid sequences were verified by Nanopore Sequencing:Article Title: Engineering plasmids with synthetic origins of replication Article Snippet: .. All assembled plasmids were verified using Construct:Article Title: Supporting Information for Visualizing spatiotemporal dynamics of intercellular mechanotransmission upon wounding Article Snippet: .. All the constructed DNA plasmids were analyzed and confirmed by restriction enzyme digestion and Transformation Assay:Article Title: Mechanism of Action of Gepotidacin: Well-Balanced Dual-Targeting against Neisseria gonorrhoeae Gyrase and Topoisomerase IV in Cells and In Vitro. Article Snippet: The quinolone resistance-determining regions of GyrA and ParC were amplified by polymerase chain reaction (PCR) using described primers.86 PCR products were amplified using Platinum PCR supermix (Invitrogen, 12532-016) and purified using a PCR cleanup kit (Qiagen, 28106). .. DNA transformation was performed according to the protocol by Dillard.82 Azenta Life Sciences was used to verify successful construction by Clone Assay:Article Title: Induced pluripotent stem cells carrying novel APTX mutations presented defective neural differentiation with the accumulation of DNA single-strand breaks Article Snippet: Positively expressed cells were selected with 1 μg/mL puromycin (Gibco, Grand Island, NY) in mTeSR Plus for 48 hours. .. The surviving cells were propagated into clones, collected, and analyzed via Recombinant:Article Title: Profiling Allogeneic HLA-specific B-cell Responses Utilizing a 64-plex Single-HLA Reporter Cell Panel Article Snippet: Double-stranded DNA fragments encoding the following genes were synthesized (Gene Universal Inc. or Twist Bioscience) based on their published amino acid sequences, including EBFP2 [ ], mTurquoise2 [ ], LSSmOrange [ ], hmKeima8.5 [ ], NowGFP [ ], mKelly1 [ ], the neomycin-resistance gene (GenBank V00618.1 , with R177S mutation), the hygromycin-resistance gene (UniProt P00557), human CD74 (GenBank NM_001025159.2), and 73 unique HLA alleles (IPD-IMGT/HLA Database [ ]) listed in . .. All recombinant plasmid sequences were verified by Plasmid Preparation:Article Title: Profiling Allogeneic HLA-specific B-cell Responses Utilizing a 64-plex Single-HLA Reporter Cell Panel Article Snippet: Double-stranded DNA fragments encoding the following genes were synthesized (Gene Universal Inc. or Twist Bioscience) based on their published amino acid sequences, including EBFP2 [ ], mTurquoise2 [ ], LSSmOrange [ ], hmKeima8.5 [ ], NowGFP [ ], mKelly1 [ ], the neomycin-resistance gene (GenBank V00618.1 , with R177S mutation), the hygromycin-resistance gene (UniProt P00557), human CD74 (GenBank NM_001025159.2), and 73 unique HLA alleles (IPD-IMGT/HLA Database [ ]) listed in . .. All recombinant plasmid sequences were verified by |